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Image Search Results
Journal: Journal of Advanced Pharmaceutical Technology & Research
Article Title: Possible carcinogenic potential of dimethyl dimethoxy biphenyl dicarboxylate in experimental animals
doi: 10.4103/2231-4040.179747
Figure Lengend Snippet: Hepatocytes of untreated (a and c) and dimethyl dimethoxy biphenyl dicarboxylate-treated (b and d) mice for 1 and 3 months, respectively (IHC, DAB, ×400), showing large number of positive cytochrome P450 (a and c) with marked intensity (brownish cytoplasmic stain) and binucleated nuclei (red arrows), and mild-moderate number of positive cytochrome P450 (b and d) with binucleated nuclei (red arrow)
Article Snippet: Feulgen stain specifically and quantitatively stains the nuclear DNA blue that can be quantified as integrated optical density,[ ] using the computerized image analysis system (Zeiss, Germany) with software program “AxioVision 4.8 (Carl Zeiss Microscopy GmbH 07745 Jena, Germany).” A number of 150–200 cells with single monolayer nuclei were subjected to DNA analysis, identified, and classified according to Eskelino et al .[ ]
Techniques: Staining
Journal: Journal of Advanced Pharmaceutical Technology & Research
Article Title: Possible carcinogenic potential of dimethyl dimethoxy biphenyl dicarboxylate in experimental animals
doi: 10.4103/2231-4040.179747
Figure Lengend Snippet: Hepatocytes of untreated (a and c) and dimethyl dimethoxy biphenyl dicarboxylate-treated (b and d) mice for 1 and 3 months, respectively (IHC, DAB, ×400), showing 10% and 15% of positive proliferating cell nuclear antigen (a and c) with strong intensity (brownish nuclear stain, black arrow) and 20–30% of positive proliferating cell nuclear antigen (b and d) with strong intensity (brownish nuclear stain, black arrow)
Article Snippet: Feulgen stain specifically and quantitatively stains the nuclear DNA blue that can be quantified as integrated optical density,[ ] using the computerized image analysis system (Zeiss, Germany) with software program “AxioVision 4.8 (Carl Zeiss Microscopy GmbH 07745 Jena, Germany).” A number of 150–200 cells with single monolayer nuclei were subjected to DNA analysis, identified, and classified according to Eskelino et al .[ ]
Techniques: Staining